Nature

Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test

The adrenaline test for enzymes is a colorimetric enzyme assay based on the quantification of periodate-sensitive reaction products such as 1,2-diols and 1,2-aminoalcohols by back-titration of the ...
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Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants

Alexandra Jammer, Anna Gasperl, Nora Luschin-Ebengreuth, Elmien Heyneke, Hyosub Chu, Elena Cantero-Navarro, Dominik K. Großkinsky, Alfonso A. Albacete, Edith Stabentheiner, Jürgen Franzaring, Andreas ...
JSTOR Daily

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

The directed evolution of enzymes for improved activity or substrate specificity commonly leads to a trade-off in stability. We have identified an activity–stability trade-off and a loss in unfolding ...