Methods The Particle-in-Cell-3D ImageJ Macro Particle-in-Cell-3D was designed to quantify the uptake of nano- and micro-particles into cells by processing stacks of images obtained via dual-color ...

Particle-in-Cell-3D is a freely available ImageJ macro. During the image analysis routine, single cells are reconstructed in 3D and split into two volumes – intracellular and the membrane region.