Real-time polymerase chain reaction (PCR), also called quantitative PCR (qPCR), is one of the most powerful tools for nucleic acid analysis. However, inconsistencies in protocols, experimental design, ...

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

Real-time PCR is an important tool for life scientists looking to evaluate gene expression. While the chemical reaction underpinning the technique is relatively simple, real-time PCR instruments can ...