Use the Excel file as an ODBC data source (connection string info here), and your MySQL database as the destination (using their ODBC connector). What OS is your process running on?

How to Create Excel Spreadsheets With MySQL. When you use MySQL to query a database, you have the ability to view results from that query on your screen or send them to a text file.

Warnings When you import a spreadsheet into an OpenOffice.org database, you lose any text styling, number formatting, macro scripts or charts that you previously used to enhance the data.