The mechanism of a confocal laser scanning microscope is to irradiate an object with a laser beam emitted from a light source, scan the entire object in two directions, the X-axis and the Y-axis, and ...
You must find out the distance measured for each division of the graticule. You can then use the graticule to measure cells. The distance will be different for each objective lens of the microscope.
Laser scanning microscopes are useful for all kinds of tiny investigations. As it turns out, you can build one using parts salvaged from a Blu-ray player, as demonstrated by [Doctor Volt]. The trick ...