The normalization of RNA-seq data is essential for accurate downstream inference, but the assumptions upon which most normalization methods are based are not applicable in the single-cell setting.

Normalization of RNA-sequencing (RNA-seq) data has proven essential to ensure accurate inference of expression levels. Here, we show that usual normalization approaches mostly account for sequencing ...

This paper considers inference for both spatial lattice data with possibly irregularly shaped sampling region and non-lattice data, by extending the recently proposed self-normalization (SN) approach ...